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This behavioral assay happened in two trials. In the first trial, Every single animal was paired that has a littermate from your household cage of the subject animal. In the 2nd demo, Each individual animal was paired which has a novel mouse with which that they had no previous Make contact with. Except animal pairing requirements, both equally phases have been executed using the identical methodology. Two mice were being positioned in an empty, plastic, sq. (twenty five × twenty five cm) arena and allowed to take a look at or interact devoid of interference. This interaction was recorded via an overhead camcorder and scored in serious-time by TopScan monitoring application (CleverSys). One particular mouse in Each individual paired trial was marked with a small dot of white-out Remedy streaked on its fur to aid the TopScan computer software in differentiating the two mice in the course of the trial.
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This behavioral assay transpired in next two trials: nonsocial pro33 maxwin and social odor detection. Within the nonsocial odor detection trial, a cotton swab was briefly dipped in a ten% condensed milk Alternative and hung from a corner of the house cage of the topic mouse for two min. All through this time, the mouse was absolutely free to check out and interact with the contents of its cage without interference. The amount of time invested within just five cm from the scented swab or instantly interacting with the scented cotton swab was recorded.
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